SELECTION, CHARACTERISTICS AND PROSPECTS OF USING PEPTIDE OF COLOSTRUM OF COWS
EDN:ARSKGW
DOI:
https://doi.org/10.25712/ASTU.2072-8921.2022.04.023Keywords:
peptides, cow colostrum, enzymes, trypsin, pepsin, hydrolysate, biological activity, specialized food products, therapeutic agentsAbstract
Despite the great successes achieved in the creation of food products for the prevention of alimentary-dependent diseases, some problems of preserving human health remain unanswered. The development of oncological diseases, bacterial and viral infections with multidrug resistance is still a threat of global importance. Natural biologically active peptides with a wide range of antimicrobial, antitumor, and immunostimulating activities are presented as an alternative to known medicinal preparations and micronutrients. The purpose of the research is to isolate, characterize and evaluate the prospects for the use of peptides of trypsin and pepsin hydrolysate of cow milk in the production of specialized food products and medicinal preparations. Protein fractions of enzymatic hydrolysate of cow colostrum were studied on a MALDI-TOPH mass spectrometer, decryption was carried out using the Mascot database, the Peptide Fingerprint option (Matrix Science, USA) using the Protein NCBI database. 7 peptides were isolated in trypsin hydrolysate of cow colostrum, 9 peptides were isolated in pepsin hydrolysate. The isolated peptides are characterized by different amino acid sequence, molecular weight, etc., and differ in physiological functions in the human body. The peptide "Dual specificity protein phosphatase" is able to activate the immune response, "NCI_CGAP_Brn23 Clone of Homo sapiens cDNA" ensures the normal development of the central nervous system, the peptide "BAMA" is able to integrate into the outer membrane of bacteria, which allows it to be used as a cargo carrier into the cells of the body to maintain vital activity or fight viral infections, to suppress tumor cells. The peptide "Nuclear receptor 2C2-associated protein" prevents the occurrence of cancer. The functions of other isolated peptides have not been studied, which suggests conducting studies to assess their biological activity. The data obtained suggest that peptides with proven pharmacological activity after additional preclinical and clinical studies can be used in the development of functional foods and therapeutic agents of a wide spectrum of action.
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Copyright (c) 2022 Sergey L. Tikhonov, Natalya V. Tikhonova, Irina G. Danilova, Anna S. Ozhgihina, Mariya S. Tikhonova, Anzhelika D. Popovskih
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