MODIFICATION OF THE PROTEIN PROFILE OF THE BIVALVE MOLLUSK MACTRA CHINENSIS

RCHYKS

Abstract

This paper presents the results of studies to substantiate the possibility of using a fraction of electroactivated water (anolyte) as a hydrolyzing agent for modifying the protein profile of soft tissues of the bivalve mollusk of the Far Eastern region Mactrachinensis. The pH of the medium, the duration of the hydrolysis process and the temperature were determined by the main studied parameters of the influence. The changes in the content of dry substances, total and amine nitrogen in the hydrolysate and the degree of hydrolysis depending on the process conditions were investigated. The hydrolysis process is most intensive for 7 hours, the dry matter content in the hydrolyzate increases by 146 % (from 2 hours to 7 hours), over the next 2 hours – by only 4 %. The content of total and amine nitrogen also maximizes with the duration of hydrolysis of 7 hours -134 % and 206 %, respectively. The degree of hydrolysis increases from 36 % at 2 hours of hydrolysis to 75 % at 9 hours. The maximum increase in the accumulation of solids, total and amine nitrogen in hydrolysates is observed at pH 3 and amounts to 14-27 % for solids, 8-10 % for total nitrogen and 11-12 % for amine nitrogen. The increase in the degree of hydrolysis is much more significant and amounts to 18-77 %. Experimentally determined that the rational parameters of hydrolysis, ensuring the achievement of the optimal content of amine nitrogen and the maximum degree of hydrolysis are pH 3, time 7 hours, process temperature 70 0C. The results of regression analysis of the established dependencies, in particular the coefficients of approximation R2, showed that the obtained quadratic equations adequately describe the regularities of the process of modification of the protein profile of soft tissues of the bivalve mollusk of the Far Eastern region Mactrachinensis depending on the pH, duration and temperature of the process.