MICROENCAPSULATION OF EXTRACTS OBTAINED FROM BRASSICA OLERACEA VAR. ITALICA SPROUTS
DSZSDR
DOI:
https://doi.org/10.25712/ASTU.2072-8921.2024.03.020Keywords:
broccoli, ultrasonic extraction, biologically active substances, phenolic compounds, flavonoids, glucosinolates, encapsulation, extrusion, functional food productAbstract
. The purpose of the study was to obtain a microencapsulated form of broccoli sprout extract optimized for the content of biologically active substances. The conditions for the extraction of phenolic compounds from broccoli were optimized according to three factors: hydraulic module, extraction duration and ultrasonic power. In accordance with the experimental data obtained, the following conditions were established: hydraulic module - 1:25; extraction duration - 60 seconds; ultrasonic power - 300 W, solvent - 70% ethanol in water. A regression equation is obtained that adequately describes the process under study. For the extract obtained under optimal conditions, the content of total phenolic compounds was determined - 482 ± 18 mg per 100 g of dried sprouts, flavonoids - 45.25 ± 2.0 mg/100 g, glucosinolates - 349 ± 16 mg/100 g, as well as antioxidant activity. The extracts were encapsulated by extrusion; the encapsulation efficiency is 95.2 ± 3.2% for phenolic compounds and 85.7 ± 2.9% for glucosinolates. The organoleptic properties of a food product (puff pastry with custard) containing a microencapsulated extract were characterized.
References
Jeffery E. H. и др. Variation in content of bioactive components in broccoli // Journal of Food Composition and Analysis. 2003. Т. 16. № 3. С. 323–330. doi: 10.1016/S0889-1575(03)00045-0
Zhang Y. и др. Induction of GST and NQO1 in Cultured Bladder Cells and in the Urinary Bladders of Rats by an Extract of Broccoli (Brassica oleracea italica) Sprouts // J Agric Food Chem. 2006. Т. 54. № 25. С. 9370–9376. doi: 10.1021/jf062109h.
Singletary K., MacDonald C. Inhibition of benzo[a]pyrene- and 1,6-dinitropyrene-DNA adduct formation in human mammary epithelial cells byd-ibenzoylmethane and sulforaphane // Cancer Lett. 2000. Т. 155. № 1. С. 47–54. doi: 10.1016/s0304-3835(00)00412-2.
Kensler T. W. и др. Effects of Glucosin-olate-Rich Broccoli Sprouts on Urinary Levels of Aflatoxin-DNA Adducts and Phenanthrene Tetraols in a Randomized Clinical Trial in He Zuo Township, Qidong, People’s Republic of China // Cancer Epi-demiology, Biomarkers & Prevention. 2005. Т. 14. № 11. С. 2605–2613. doi: 10.1158/1055-9965.EPI-05-0368.
Ritz S. A., Wan J., Diaz-Sanchez D. Sul-foraphane-stimulated phase II enzyme induction inhibits cytokine production by airway epithelial cells stimulated with diesel extract // American Journal of Physiology-Lung Cellular and Molecular Physiology. 2007. Т. 292. № 1. С. L33–L39. doi: 10.1152/ajplung.00170.2006.
Canene-Adams K. и др. Combinations of Tomato and Broccoli Enhance Antitumor Activity in Dunning R3327-H Prostate Adenocarcinomas // Cancer Res. 2007. Т. 67. № 2. С. 836–843. doi: 10.1158/0008-5472.CAN-06-3462.
Bosetti C. и др. Micronutrients and the risk of renal cell cancer: A case‐control study from Italy // Int J Cancer. 2007. Т. 120. № 4. С. 892–896. doi: 10.1002/ijc.22374.
Hashem F. и др. Myrosinase hydrolysates of Brassica oleraceae L. var. italica Plenck // Planta Med. 2011. Т. 77. № 12. doi: 10.1002/ptr.3591.
Talalay P. и др. Sulforaphane mobilizes cel-lular defenses that protect skin against damage by UV radiation // Proceedings of the National Acade-my of Sciences. 2007. Т. 104. № 44. С. 17500–17505. doi: 10.1073/pnas.0708710104.
Owis A.I. Broccoli; The Green Beauty: A Review // J. Pharm. Sci. & Res. 2015. Т. 7. № 9. С. 696–703.
Cartea M. E., Velasco P. Glucosinolates in Brassica foods: bioavailability in food and signifi-cance for human health // Phytochemistry Reviews. 2008. Т. 7. № 2. С. 213–229. doi: 10.1007/s11101-007-9072-2.
Axelsson A. S. и др. Sulforaphane reduc-es hepatic glucose production and improves glu-cose control in patients with type 2 diabetes // Sci Transl Med. 2017. Т. 9. № 394. doi: 10.1126/scitranslmed.aah4477.
Mohammed A., Mohammed H. A. Benefi-cial role of broccoli and its active ingredient, sul-foraphane in the treatment of diabetes // Phytomed-icine Plus. 2023. Т. 3. № 2. С. 100431. doi: 10.1016/j.phyplu.2023.100431.
Ares A. M., Nozal M. J., Bernal J. Extrac-tion, chemical characterization and biological activi-ty determination of broccoli health promoting com-pounds // J Chromatogr A. 2013. Т. 1313. С. 78–95. doi: 10.1016/j.chroma.2013.07.051.
Хуссайне Р., Сучкова Е.П., Арсеньева Т.П. Оптимизация условий экстракции для извлечения фенольных соединений и антиоксидантов из ароматических растений // Вестник Международной академии холода. 2024. № 2. С. 58-63. DOI: 10.17586/1606-4313-2024-23-2-58-63.
Способ определения глюкозинолатов в семенах крестоцветных: пат. SU 1406481 A1; опубл. 24.04.1984.
Способ определения антиокислительной активности: пат. RU 2 170 930 C1 заявл. 05.05.2000; опубл. 20.07.2001.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Elmira R. Eminova, Angelina S. Sorokina, Ludmila A. Zabodalova, Nikita Tyutkov, Pablo Alloh, Denis A. Baranenko
This work is licensed under a Creative Commons Attribution 4.0 International License.