DEVELOPMENT OF A MULTIENZYMATIC COMPOSITION FOR BREWER’S SPENT GRAIN HYDROLYSIS THROUGH USE OF MATHEMATICAL MODELING METHODS

KSGYGV

Abstract

The study was focused on preparing a multienzymatic composition for brewer’s spent grain hydrolysis through the use of mathematical modeling methods. The object of the study was spent grain obtained through traditional brewery. Complex cellulolytic, xylanasic, β-glucanasic and proteolytic enzyme preparations produced by Production Association Sibbiopharm Ltd. (city of Berdsk) were used for enzymolysis: Cellolux-A, Cellolux BGK, Protosubtilin. In the experiment, spent grain was mixed with water at the temperature of 51±2 °С at a 1:5 ratio. Upon introducing the enzyme preparations, the resulting suspension was maintained at the temperature of 50 °С with intermittent mixing in the course of six hours. Samples, which were collected hourly, underwent centrifugation and the supernatant fluid was used to determine the concentration of reducing sugars and amino nitrogen. As a result, the dosages of enzyme preparation introduction have been determined, the values of which were used to design and conduct a full factorial experiment FFE 2⁴ with the following factors: the duration of hydrolysis and the dosage of each of the three enzyme preparations. On the basis of the results of a series of experiments, two regression equations which described the processes of reducing sugars and amino nitrogen accumulation, with both factors in co-operation, were deduced. The resulting mathematical models were shown to be adequate by passing the F-test and were tested experimentally, with the values determined not exceeding the observational error. As a result of analyzing the deduced correspondences, the factor ratios which achieve the most efficient co-accumulation of reducing sugars and amino nitrogen were specified.